The article presents the history of the Small Seminary of Vincentian Fathers in Vilnius in the interwar period. The institution has its roots in the 17th century, in the times of St. Vincent de Paul. The first Small Seminaries administered by Missionaries appeared in Poland only in the times of partitions, at the beginning of the 19th century in the Province of Lithuania. In the Province of Cracow a Small Seminary was created in 1878. In the 1920s a great number of candidates applied to the Cracow Seminary, that being the reason why the Vincentian Fathers decided to create a second institution of this type in Vilnius, in the school year 1924/25. Its dual aim was to reduce the number of applicants in the Cracow institution as well as to facilitate the access to the seminary for students from Easter Poland. The Small Seminary of Vincentian Fathers was a private educational institution for young people who wanted to dedicate themselves to priesthood. The institution comprised four lower grades of secondary school according to the programme of state gymnasiums of classical type. With the help of their superiors, Seminary students had a possibility to recognize their calling and qualifications for future work in the Congregation on missions or in formative work in diocesan seminaries. Others – through learning in male gymnasium of the Congregation – could benefit from the comprehensive development guaranteed by the school as a way of preparation for social life or undertaking university studies of various branches.