The article was based on archival materials and contemporary publications. Most of the relevant sources are located in the Archives of the Province of the Daughters of Charity in Krakow, other important records are located in individual houses of the Congregation that were established in 19th-century Galicia. Because the Sisters of Charity worked in completely different conditions and environments, this description of their mission is divided into three parts. The first part is about working in a hospital. The sisters were employees of fourteen private facilities in small towns and villages, financed by charitable foundations, twenty-two public hospitals in larger cities of Galicia and three specialized facilities (for psychiatrists, children and a military hospital). In total, 348 Daughters of Charity worked in all types of hospitals during this period. The second part concerns the work of the Daughters of Charity in homes for the terminally ill and the elderly (Kraków, Lviv, Czortków, Stryj), their care for patients in the outpatient departments of the Houses of Congregation and visits to the homes of the sick. The number of visits ranged from 3,465 in 1870 to 6,466 in 1881. Hot lunches were served to the poor every day in the Central Provincial House in Kraków. The last part of the article concerns the work of the Daughters of Charity in the women's prison in Lviv (care, professional preparation, upbringing, supervision during work) and in two homes for orphans in Lviv and Krakow. In their homes throughout Galicia, the Daughters of Charity provided shelter and education for girls from poor families.